Page 84 - PG 504-theoretical notes-phyto-1-2024-2025..

Clinical pharmacy PharmD program Third level Phytochemistry-1 (PG-504)

• The clarified decolorized liquor is concentrated in vacuum and crystallized.

Inversion of sucrose:

On hydrolysis by acid or enzyme of yeast (invertase), it yields

equimolecular quantities of D-glucose and D-fructose (both in the pyranose

form). This mixture is known as invert-sugar, and the process is called

“Inversion” since the optical rotation changes from dextro to levo. As the

laevorotation due to D-(-)-fructose exceeds the dextrorotation due to D-(+)-

glucose, the hydrolysis results in change in sign of rotation.

Sucrose + Water Acid or D-(+)-Glucose + D-(+)-Fructose
Enzyme
+ 66.5 O + 52.2 O - 93 O Specific
Invert sugar = - 20.4 O rotation

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