Page 9 - PG 504-theoretical notes-phyto-1-2024-2025..

Practical
Week
No. Practical Topics credit
hours
*Qualitative estimation of monosaccharides & non-reducing disaccharides
(Glucose. Fructose. Sucrose)
1. *Qualitative estimation of reducing disaccharides, pentoses, and polysaccharides 1
) Maltose, lactose, Xylose, Rhamnose, Starch)
* General scheme of carbohydrates
*Quantitative estimation of carbohydrates (glucose)
2. 1
-Copper reduction method
*Quantitative estimation of glucose and fructose mixture
3. -Copper reduction method 1
-iodometric method
Qualitative identification of glycosides (Anthraquinones) Quantitative estimation of
4. 1
anthraquinone
5. First Practical Exam (sheet/spots) 1
Qualitative identification of glycosides (Cardiac glycosides) Colorimetric estimation
6. 1
of digitalis glycosides by Baljit’s reagent

Qualitative identification of glycosides (Flavonoids, Cyanogenic and saponin
7. glycosides) 1
Qualitative and Quantitative identification of bitter principles (Khellin)
Extraction of volatile oil by steam distillation.
8. 1
-Determination of eugenol in clove oil.
9. Quantitative estimation of cinnamaldehyde in Cinnamon oil 1
Quantitative estimation of nitrogenous and sulfur volatile constituents (e.g., allyl
10. 1
isothiocyanate in mustard oil.).
11. Determination of peroxides in oil of chenopodium. Determination of oxides (in cineol). 1
12. Preliminary phytochemical screening of herbal drugs 1
13. Revision and evaluation 1

14. Final Practical Exam (Practical scheme). 1