Page 86 - ASME_NEMB_2016_Program

Technical Program TRACK 6

We developed a web-based image processing application for automated Flow Controllable Lateral Flow Device
and objective quantification of HemeChip results at the POC using cloud
computing resources. This intensity-based mobile phone image quantitation Poster Presentation. NEMB2016-6108
method showed high correlation with HPLC results for tested patient blood
samples (PCC=0.95). Moreover, the Bland-Altman analysis showed strong Yuxin Liu, Veronica Betancur, Nianqiang Wu, West Virginia Uni-
agreement between the HemeChip results analyzed with the mobile user
interface and HPLC. The majority (91%) of the differences between actual versity, Morgantown, WV, United States
(HPLC) and estimated (mobile user interface) were within limits of agreement.
Lateral flow devices have been used and are promising in many applica-
HemeChip technology offers a low-cost, easy to use, rapid approach and tions, including point of care diagnosis in medical, clinical, food and environ-
an innovative solution to POC diagnosis of SCD and other hemoglobin dis- mental control and monitoring. In particular, disposable paper-based lateral
orders. HemeChip can distinguish between different patient phenotypes, flow strips utilize low-cost materials and do not require expensive fabrication
including HbSS (HbS only), transfused HbSS (HbS and HbA), and Hemoglo- instruments. However, there are constraints on tuning the flow rate and
bin SC disease (HbS and HbC). In summary, the HemeChip identification immunoassays functionalization in paper as well as technical challenge in
and quantification of hemoglobin phenotypes, as a POC technique, were integration of sensors and concentration units for low abundant molecular
comparable to standard clinical methods. This platform has clinical potential detection. In the present work, we demonstrated an integrated lateral flow
in under-served populations worldwide, in which SCD is endemic. device that applied the capillary forces with functionalized polymer-based
microfluidics as a strategy to realize a portable, simplified, and self-powered
lateral flow device for potentially biomarker detection with minimizing the
need for off-chip equipment. The device combined the ability to separate
One-touch-activated Blood Multidiagnostic System Using a Hol- plasma from human whole blood and create a controlled and steady flow.
low Microneedle Integrated with a Paper-based Sensor Lateral flow relies on surface tension and hydrophilic natural of the flow
channel, and controlled flow is also a key variable for immunoassay based
Poster Presentation. NEMB2016-6098 applications for providing enough time for protein binding to antibodies. To
render polydimethylsiloxane (PDMS) surface of hydrophilicity as well as to
Chengguo Li, Yonghao Ma, Manita Dangol, Hyungil Jung, Yonsei control the flow rate, PDMS was functionalized with different concentrations
University, Seoul, Korea (Republic) of Pluronic F127. The results show that, in an integrated LFD, the flow rate
was regulated by the combination of multiple factors, including Pluronic F127
functionalized surface properties of microchannels, resistance of the inte-
Point-of-care testing (POCT) represents a system whereby simple, rapid, and
low-cost medical diagnostic tools are used at the patient care sites, and has grated flow resistor, the dimensions of the microstructures in the capillary
progressed dramatically in the last few years with advances in biomedical pump, dynamic contact angles, and viscosity of the fluids. The predominant
microelectromechanical systems, lab-on-a-chip devices, and micro total factors are the resistance of the integrated flow resistor and the surface
analysis systems. Blood, a rich source of human biological information, is hydrophilicity of Pluronic functionalized PDMS, which can be easily modified
by the dimensions of the flow resistor and the Pluronic concentrations as
used as the target diagnostic sample in various POCT biosensors such as
glucose meters, lateral flow assay strips, and cholesterol meters. However, well as post-functionalization treatment, respectively. The flow rates ranging
the separation of blood collection and sample injection in commercially between 0.5 and 18.6 nL/s were achieved. In addition, the highly asymmetric
available POCT devices has been shown to negatively influence the perfor- nature of a plasma separation membrane allowed the cellular components
mance of biosensors. In addition to this, the use of hypodermic needles or of the blood to be captured in the larger pores without lysis, while the
plasma flowing down into the smaller pores on the downstream side of the
finger pricking for blood collection and use of pipettes or disposable drop-
pers for sample injection limit the miniaturization of the POCT system, result- membrane was driven into the lateral flow device by the capillary pump. The
ing in patient inconvenience. Although the creation of an all-in-one POCT integrated lateral flow device required no external macroscopic actuators
system by combining blood collection with biosensor components has been and bulky fluidic connections for initiating and controlling fluid flow, demon-
a longstanding goal for complete, real-time blood diagnosis, such a system strated the capability in using of whole blood, showed the potential for
future diagnostic applications in home and point of care situations with low
has not yet been developed.
cost, small sample volume and portability.
To become a useful tool in an all-in-one POCT blood diagnostic system,
an improved miniature system must reduce the actuator complexity while
simultaneously simplifying the operation process. Currently, paper-based Determining the Influence of Dynamic Paracrine Signaling on
biosensor technology has been recognized as a future alternative for POCT Tumor Progression in an Evolving Microenvironment
biosensors for detection and quantification of a broad variety of analytes
due to the high specificity and sensitivity of these biosensors as well as their Poster Presentation. NEMB2016-6101
simple and cost-effective fabrication process. Various paper-based POCT
biosensors such as rapid kits, dip sticks, and glucose meters are already Manasa Gadde, University of Texas at Austin, Austin, TX, United
commercialized and allow portable, on-site detection based on colorimetric
methods, however, almost all of them are separated from the crucial sample States, M Nichole Rylander, Univeristy of Texas at Austin, Austin,
collection process. TX, United States

Here, we demonstrate a novel one-touch-activated blood multidiagnostic Cancer research aimed at developing anti-angiogenic therapies is limited by
system (OBMS) that consists of a biocompatible minimally invasive hollow a lack of physiologically representative platforms that can be used to study
microneedle and paper-based multiplex biosensor. We took advantage of the dynamic intercellular interplay and tumor stromal interactions within an
microneedle technology for pain-free blood sampling with minimal tissue evolving microenvironment. Greater understanding of the role of key players
damage and inflammation, and paper-based sensors that have a simple and in the process of angiogenesis will lead to the development of improved
cost-effective fabrication process. The OBMS integrated and automated treatments that will halt cancer progression. The objective of this study
all functions of blood collection, separation, and detection, sequentially, was to increase the complexity of an existing 3D in-vitro microfluidic tumor
in a single device that only required one-touch activation by finger-power model comprised of a co-culture of microvascular endothelial cells and
without additional operations. In an in vivo experiment, using a rabbit model breast cancer cells with the incorporation of fibroblasts, a key component of
we demonstrated that the OBMS could perform blood sampling and mul- the tumor stroma. This model was used to investigate the influence of fluid
tidetection of glucose and cholesterol levels in a fully automated manner. shear stress and paracrine signaling between these cells on the expression
86 This new integrated microneedle and paper-sensor based blood diagnostic of pivotal markers of angiogenesis including Matrix Metalloproteinase 9
platform shows great promise for future POCT and disposable biomedical (MMP-9), Angiopoietin-1 (ANG-1), Angiopoietin-2 (ANG-2), and Vascular endo-
applications. thelial growth factor (VEGF). In-vitro tumor models were constructed using