Page 90 - ASME_NEMB_2016_Program

Technical Program TRACK 6

filopodia were observed surrounding each cell, suggesting the probing of (Quantel USA) and the protein inactivation was measured by a colorimetric
the array to find new adhesion points. Overall, the iron nanowire array is a enzyme assay. The experiment shows that the protein inactivation is depen-
potential platform for studying stem cell differentiation, as well as to influ- dent on laser pulse energy and complete inactivation (up to 90%) is pos-
ence it through magnetic field mechanical stimuli. sible. When the enzyme is not attached to the protein, no inactivation was
observed, suggesting a highly selective and localized effect.

Protein-lipid synergy drives mitochondria fission In summary, this study proves the selective protein inactivation adjacent to
gold nanoparticles during nanosecond pulsed laser heating and establish-
es a numerical and experimental approach to investigate and understand
Poster Presentation. NEMB2016-6075 this important phenomenon. The protein inactivation can be controlled in
the nanometer scale by laser energy, nanoparticle size, distance between
Ehsan Irajizad, University of Houston, Houston, TX, United States, protein and nanoparticle, and thermal interface resistance. Furthermore, our
Rajesh Ramachandran, Case Western Reserve University School study has a broader impact in understanding the nano-bio interface involv-
of Medicine, Cleveland, OH, United States, Ashutosh Agrawal, Uni- ing laser nanoparticle heating, and designing novel nano-surgical tools to
versity of Houston, Houston, TX, United States selective inactive proteins of interest in living systems.

Mitochondria are one of the most important organelles in our cells and are
responsible for breathing of the cell and supplying energy for our body. How 6-5
they maintain their shape and undergo fission, however, continues to remain NANOMATERIALS DESIGN AND MANUFACTURING
a mystery. Experimental studies have implicated various proteins and lipids
such as Cardiolipin, Drp1, PE, Fis1 in fission but the energetics and pathway
for mitochondria fission still remain elusive. In this study, we simulate pro- Grand Ballroom 5:00pm - 8:00pm
tein-lipid interactions and provide novel insights into mitochondrial fission.
Our study reveals that the mitochondrial fission is driven by an instability
triggered by DRP1 proteins and PE lipids. The computed shapes from our
simulation match closely with the shapes observed experimentally. Plasmonic tweezers for biology and medicine: towards low-pow-
er and versatile manipulations of cells and biomolecules
Numerical and Experimental Investigation of Gold Nanoparticle Poster Presentation. NEMB2016-6067
Heating-induced Protein Inactivation for Nano-Bio Interface Ap-
plications Linhan Lin, Xiaolei Peng, Yuebing Zheng, The University of Texas
at Austin, Austin, TX, United States
Poster Presentation. NEMB2016-5976
Versatile, non-invasive manipulation and patterning of cells and biomol-
Peiyuan Kang, Oumar Fall, The University of Texas at Dallas, ecules play a critical role in early disease diagnosis, medicine, tissue en-
Richardson, TX, United States, Jaona RANDRIANALISOA, GRESPI gineering, and fundamental studies in life sciences. Despite its capability
University of Reims Champagne-Ardenne, Reims,France, Zhenpeng of offering remote, real-time and versatile manipulations of particles, con-
Qin, University of Texas at Dallas, Richardson, TX, United States ventional optical tweezers require high laser power and have remained
challenging in the non-invasive operation. Employing the near-field effect of
the surface plasmons and its capability to concentrate light at the subwave-
Gold nanoparticles (GNP) have received broad interest in biomedical appli-
cations due to their unique optical and thermal properties. Specifically, GNP length scale, we develop a new type of plasmonic tweezers to non-invasive-
ly manipulate and pattern cells and biomolecules on plasmonic substrates
can be designed to strongly absorb or scatter optical energy at different by low-power laser beams. The plasmonic substrate is optimized to match
wavelengths of interest. The laser energy absorption can lead to significant the plasmonic resonance wavelength with the laser wavelength and have
temperature changes on the nanoparticle as well as the surrounding bio- high-density “hot spots”, which improve the optical force and dramatically
logical environment. Among the molecular components in the biological
system, proteins are extremely thermally sensitive and play a critical rule in reduce the laser power. Through directing the laser beam, dynamic trapping
and versatile manipulation of single/multiple biological particles is achieved.
performing biological functions. Although the thermal effect on proteins are Furthermore, employing the plasmon-enhanced photothermal effect, a mi-
well understood in low temperatures (below 100°C) and longer time scale crobubble is generated to capture and immobilize the biological particles on
(seconds to minutes), the fate of protein under short time scales (enabled by the substrate through coordinated actions of Marangoni convection, surface
nanosecond pulsed lasers) are less understood.
tension, gas pressure, and substrate adhesion. Arbitrary patterns of particles
and cells with different architectures are demonstrated. With the low-power
In this research, we investigated on the effect of GNP heating on proteins in operation, versatility, and biocompatibility, this new plasmonic tweezer will
the nanosecond and nanometer scale. Firstly, we performed numerical simu- find a wide range of applications in biology and medicine.
lations to obtain the GNP optical absorption, thermal responses and protein
inactivation, by using the Mie theory, heat equation and Arrhenius model,
respectively. The protein inactivation was found to be strongly dependent
on the laser pulse energy, GNP size, distance between the protein and GNP, Moire metasurfaces: from nanoengineering to applications in
and pulse number. However, the protein inactivation is independent of the biology and medicine
laser pulse shape (Gaussian versus Rectangular with similar pulse width)
and temperature dependent thermal properties of water. Thermal interface Poster Presentation. NEMB2016-6058
conductance (TIC) significantly increases the GNP temperature but exerts a
relatively smaller effect on surrounding water due to the large specific heat Yuebing Zheng, Yuebing Zheng, The University of Texas at Austin,
of water. TIC was found to significantly reduce the heat flux from GNP to
water and thus affect protein inactivation when the value is smaller than 100 Austin, TX, United States
MW/(m2 K).
Moiré metasurfaces consist of arrays of plasmonic nanostructures in moiré
90 Secondly, protein inactivation was studied experimentally by conjugating an patterns. Due to the high rotational symmetry and a wide arrange of nano-
structure sizes and shapes, moiré metasurfaces exhibit unique optical
enzyme (alpha-chymotrypsin) to 15nm GNP with a polyethylene glycol (PEG)
spacer. GNP was heated by an Nd:YAG nanosecond pulsed laser at 532nm property that can be harnessed for various applications. Herein, we report
our recent progress in fabrication, characterization and applications of moiré