Page 91 - ASME_NEMB_2016_Program

TRACK 6 TRACK 6 Technical Program

metasurfaces in biology and medicine. Firstly, we have developed moiré staining. From the results, it was demonstrated that the fabricated mat with
nanosphere lithography (MNSL) for the high-throughput and cost-effective hybrid structure could provide significantly improvement in cell migration as
fabrication moiré metasurfaces. We create moiré metasurface patch by well as cell viability.
stacking a thin Au moiré metasurface layer on an optically thick Au layer
separated by a dielectric spacer layer. Secondly, we have employed both This work was supported by the National Research Foundation of Ko-
experiments and simulations to show that the moiré metasurfaces and their rea(NRF) funded by the Ministry of Science, Republic of Korea. (No. NRF-
patches exhibit multiple and tunable plasmonic modes. For example, the 2015R1A2A2A01005515)
moiré metasurfaces can have the strong absorption (>>95%) of incident light
across a broad range of electromagnetic spectrum, i.e., near-infrared (NIR)
range from 900 nm to 1700 nm and mid-infrared (MIR) range (~5 um). The Development of a Low-Cost Electron Paramagnetic Resonance
multiband responses and strong field enhancements of the moiré metasur- (EPR) Spectrometer to Measure Properties and Heating of
faces patches are exploited for dual-functional applications in nanospectros- Iron-Oxide Nanoparticles
copy and phototherapy. Lastly, we have demonstrated that the moiré meta-
surfaces exhibit chiral optical responses in a wide range of wavelengths due
to the rotational symmetry of the metasurfaces. The optical chirality under- Poster Presentation. NEMB2016-6022
pins a new type of optical sensors that can detect the chirality of molecules
for applications in nanomedicine and life sciences. Kalkidan A. Molla, Gregor Adriany, Michael Garwood, University
of Minnesota, Minneapolis, MN, United States

Fabrication of hybrid fibrous structure using drum collec- Abstract:
tor-based electrospinning Iron oxide nanoparticles (IONPs) are in development in laboratories through-
out the world for their capabilities in diverse biomedical applications.1 IONPs
are designed for multifunctional uses, including providing contrast agent in
Poster Presentation. NEMB2016-6123
imaging and for delivering therapies. Despite their potential for such applica-
tions, much remains to be learned about their physical properties, biocom-
Jeong Hwa Kim, Department of Mechanical Engineering, Gradu- patibility, and effectiveness for treating cancers using magnetic fluid hyper-
ate School, Kyungpook National University, Daegu, Other, Korea thermia (MFH). In this project, we are designing and building a new tool for
(Republic), Young Hun Jeong, School of Mechanical Engineering, studying IONP properties and MFH heating. Specifically, we are building a
Kyungpook National University, Daegu, Korea (Republic) low-cost electron paramagnetic resonance (EPR) spectrometer using only a
radiofrequency field.2 In our studies of IONPs, this system will be used for
Nanofibers are very useful in biomedical engineering and filtration fields two purposes: First, we will use it to measure the EPR spectrum of different
because they have various advantages such as high surface area to volume IONP formulations. Second, we will use our device to investigate the pos-
ratio, porosity, and good permeability. As a result, diverse kinds of nanofi- sibility to increase MFH heating using circularly-polarized radiofrequency
bers have been introduced to various applications of tissue engineering and irradiation to modulate the direction of the magnetization (as opposed to the
cell biology. Electrospinning is known as one of the most typical methods to conventional linearly-polarized RF field).
fabricate nanofibers, however, electrospun nanofibers have strong limitation
in cell migration and porosity control. As a result, the seeded cells mainly References:
covers a fibrous mat instead of going into inside of the mat. Thus, it is very 1. Lodhia, J et al. Development and Use of Iron Oxide Nanoparticles (Part 1):
difficult to find the cell migration over full thickness of fibrous mat. Synthesis of Iron Oxide Nanoparticles for MRI. Biomedical Imaging and Inter-
vention Journal 6.2 (2010): e12. PMC.
In this study, we present a hybrid fibrous structure enhancing controllability 2. G. Whitfield and A. G. Redfield, Physical Review, 1957, 106, 918.
of porosity as well as cell migration. The hybrid fibrous structure is fabricated
using a drum collector electrospinning. The polymer for electrospinning was
polycaprolactone with number average weight of 70,000 - 90,000. Its solu- Antibacterial Activity of Cationic-cellulose Nanofibers treated
tions with various concentration were prepared after dissolving in pure chlo- with Zataria multiflora Boiss. Extracts
roform for 12 h. Also, a drum collector based electrospinning equipment was
prepared to fabricate various types of electrospun fibers by varying process
parameters including rotation speed of drum. It was equipped with a syringe Poster Presentation. NEMB2016-5901
pump, high voltage power supply, and a servo motor for rotating drum with
higher velocity controllability as well as high speed. In the experiments, PCL Soudabeh Hajahmadi, Najafabad branch, Islamic Azad University,
fibers with variety of diameters, thickness and morphologies were fabricated Esfahan, Esfahan, Iran
under various electrospinning and drum speed conditions.
Cellulose-based textile have been widely used by consumers due to its
The morphologies of the electrospun fibers were observed using a outstanding properties such as no static electricity, biodegradability, good
field-emission scanning electron microscopy. From the observation results, absorbency, comfort soft hand , breathability and hygroscopicity. For these
it could be seen that the diameter of fibers changed with good consistency reasons by discovering nanofibers in textile industry cellulose Nanostruc-
between 700 nm and 3 um according to the solution concentration and tip tures fiber also grab worldwide considerable attention besides, amongst
to collector distance. Also, it was shown that the level of fiber alignment all nanofibers, cellulose-based nanofibers are very fascinating due to easy
could be controlled using drum speed. Also, the thickness of fiber mat was fabrication via the electrospinning process, it is well known that cationizing
controlled using electrospinning time. As a result, three types of mat with cellulose by the cationic agent shows significant advantages in terms of
different combinations of fiber diameter and thickness were fabricated to improved dyeing properties because the repulsive charges on cellulose
investigate the cell migration into the mats. surface can eliminate with surface modification of cellulose by using cationic
agents.
To indicate cell viability and mobility of the mat, in-vitro cell tests using fi-
broblast cell line (NIH-3T3) were performed. Cells were cultured in medium On the other hand, Antimicrobial textiles are becoming very important and
supplemented with 10% fetal bovine serum and 1% penicillin streptomycin demandable due to different health problems and global environmental
under the typical culture conditions (humidified atmosphere of 5% CO2 in air awareness issues.
at 37 °C). Cells were seeded at a concentration of 10,000 cells/sample, and
then the media in each samples was replaced with fresh media at every two The present study was carried out to determine the antimicrobial properties 91
days for 7 days. Cell proliferation was assessed using DNA content in each of cationic-cellulose nanofibers which produces by electrospinig process
mat. The cell morphology was assessed using SEM images and F-actin/DAPI then dyed with Zataria multiflora Boiss extracts( asa a bioactive antibacterial