Page 92 - ASME_NEMB_2016_Program

Technical Program TRACK 6

agent), with cold pad batch method as an ecofriendly dyeing method, with gold nanostructures. The morphological differentiation was fundamentally
different dye concentration. driven by incubation temperature, time, and environmental pH. Differentiat-
ed morphologies varied from dendritic, snowball, to polyvillic. This differenti-
Zataria multiflora Boiss. is a thyme-like plant belonging to the Lamiaceae ation approach initiates a myriad of shapes using relatively low temperatures
family with a remarkable antibacterial and antioxidant activity. This plant with in aqueous solution wherein the branching can be finely tuned. The extent
vernacular name of Avishan Shirazi (in Iran) has traditional uses such as anti- of branching, size of nanostructure and morphology found to be facilitating
septic, anesthetic and antispasmodic and a popular spice in food or drinks. cellular internalization with significant loading which could be detected by
Nanofiber webs dyed with Zataria multiflora Boiss. extracts by padding bright field imaging, and Raman spectroscopy. Origin of near infrared absor-
method and the different dye concentrations for padding were selected (40 bance in these nano-differentaiations enunciate their probable use in broad
and 60 gram per litter). The color strength (K/S value) of all dyed swatches spectrum of biomedical uses ranging from in vitro diagnostic to tissue pene-
was evaluated by using UV/Vic spectrophotometer. trating detection agents.
The antimicrobial activity of samples was considered against gram negative
(Pseudomonas aeruginosa) and gram positive (Staphylococcus aureus) bac-
teria. Fabrication of an Electrospun Chitosan-blend-Poly(Ethylene
Oxide)-co-Fibrinogen Nanofibrous Scaffold with Biphasic Drug
Antibacterial properties of cationic-cellulose nanofibers were estimated by
standard AATCC Test Method 100-2004, all fabric swatches were sterilized Delivery Potential
before antimicrobial testes by U.V autoclave, the dilution medium was nu-
trient broth to evaluate the antimicrobial activities, the reduction in colony Poster Presentation. NEMB2016-5915
number between the treated and untreated nanofiber webs after incubation
was specified. Phillip M. Jenkins, Tony T. Yuan, Naval Medical Research Unit San
Antonio, JBSA Fort Sam Houston, TX, United States, Ann M. Di-
All Dyed species illustrated very effective antimicrobial properties, more George Foushee, Naval Medican Research Unit San Antonio, JBSA
than 95% microbial reduction in all bacterial population. In higher concentra- Fort Sam Houston, TX, United States, Angela R. Jockheck-Clark,
tion of Zataria multiflora Boiss. the antibacterial activity of samples increased Jonathan M. Stahl, Naval Medical Research Unit San Antonio,
remarkably. The obtained results show that the antimicrobial activity of nano-
fibers is due to the presence of Zataria multiflora Boiss therefore, Zataria JBSA Fort Sam Houston, TX, United States
multiflora can be used in eco-friendly dyeing and antimicrobial finishing of
Cationic-cellulose Nanofibers, this colored textile with breathable and hy- Introduction:Electrospinning is a process that allows for a great deal of ver-
gienic properties can potentially be considered as future apparel for surgical satility in producing novel biomaterials and can be used to generate nano-
gowns or for other hospital usages, institutional articles, casual and fashion. fibrous scaffolds. Electrospun nanofibrous scaffolds can be prepared with a
high degree of structural control, thereby generating properties needed for
biological success. The objective of this study was to fabricate chitosan (CS)
and fibrinogen (FI)-based bioactive nanofibrous scaffolds, and to evaluate
Secure IPN’s Nano Fibers for Insulin Delivery
the drug loading potential, degradation, and biological and physical prop-
erties. The ability to electrospin nonwoven CS and FI-based nanofibers de-
Poster Presentation. NEMB2016-6105 signed for controlled drug delivery is an important step in the development
of a stable natural polymer-based wound dressing.
Hadi Al-Lami, Basrah, Basrah, Iraq, Zainb Sweah, Athir Haddad,
University of Basrah, Basrah, Iraq Methods: CS-based scaffolds were fabricated as a blend of CS with poly(eth-
ylene oxide) (CS/PEO) at a 2:1 weight ratio, while FI-based scaffolds were
SUMMARY fabricated as a homogenous solution. Both scaffolds were electrospun using
Novel triblock copolymers of poly(L-lactide)-poly(ethylene glycol)-seba- a stationary plate electrospinning apparatus. CS/PEO-FI scaffolds were fab-
cate-poly(ethylene glycol)-poly(L-lactide) were synthesized by Ring-Opening ricated by co-electrospinning the two systems via a dual spinneret/mandrel
Polymerization (ROP) using Diazabicyclo-[5.4.0]- undec-7-ene (DBU) as apparatus. The morphology of the scaffolds was characterized by scanning
catalyst and different ratio of L-lactide with pre-prepared poly(ethylene gly- electron microscopy (SEM). Scaffold degradation was evaluated for up to 48
col)-sebacate-poly(ethylene glycol) polymers, namely poly(ethylene glycol) hours in cell culture media at 37°C by dry weight and Fourier Transform Infra-
molecular weight, 2000, 4000, and 10000 g/mole respectively. These poly- red (FTIR) spectroscopy. Platelet-derived growth factor (PDGF) was loaded
mers were loaded with 50 IU insulin, and then they were crosslinked with into both electrospinning solutions and the release kinetics for CS/PEO and
hydroxyl ethyl cellulose to produce the nanofiber IPN’s samples. The results FI nanofibrous scaffolds assessed by ELISA. Finally, fibroblast viability and
simply supported that loading of insulin with prepared nanofiber IPN’s can proliferation were evaluated using water-soluble tetrazolium (WST-1) assay to
be used for controlled insulin release. test biocompatibility.
Results: Evaluation of SEM micrographs showed randomly aligned CS/
PEO (2:1), FI, and CS/PEO-FI nanofibers with a mean fiber diameter of 244
POLYVILLIC NANOSTRUCTURES FOR BIOMEDICAL APPLICA- ± 70 nm, 488 ± 86 nm, and 353 ± 133 nm, respectively (n = 100). Scaffold
TIONS: A SNOWFLAKE INSPIRED DESIGN degradation indicated a biphasic degradation of CS/PEO, in which PEO was
degraded in a burst release. In contrast, FI degradation indicated a steady
Poster Presentation. NEMB2016-5913 and sustained release of FI over 48 hours, and CS/PEO-FI scaffolds showed
characteristics of both individual scaffolds. Chitosan/PEO released PDGF as
Aaron Schwartz-Duval, Santosh Misra, Ayako Ohoka, Fatemeh predicted by degradation results, in a burst release within the first few hours.
Ostadhossein, Jasleena Singh, Dipanjan Pan, University of Illi- Fibrinogen PDGF release was sustained over 48 hours. None of the three
nois, Urbana, IL, United States scaffolds significantly impacted fibroblast viability or proliferation over five
days.
Gold has continued to be of scientific interest throughout the majority of Conclusion: CS/PEO- and FI-based scaffolds are biocompatible, have fa-
mankind’s existence. Initially for its luster, used as currency and in recent de- vorable degradation kinetics, and are capable of being co-electrospun with
cades gold has been found to have many other applications in electronics growth factors such as PDGF. Furthermore, these studies demonstrate the
and medicine. At the nanoscale, gold has been applied toward drug deliv-
92 ery, diagnostic imaging contrast enhancement, and photothermal ablative feasibility of fabricating co-electrospun CS/PEO-FI nonwoven nanofibrous
therapies. We describe our preliminary findings on a novel synthetic meth- scaffolds. Together, these studies demonstrate that CS/PEO and FI scaffolds
are excellent candidates for future drug-release applications, and suggest
odology initiating morphological differentiation of highly controlled branched