Page 52 - Effects of dried citrus pulp on nutrient intake, nitrogen balance and growth performance in Lohi lambs

(v/v) and 10% of it was sampled and frozen. At the end of each collection period, preserved
urine samples were composited after thawing, and 10% of the composited sample was used

for analysis (Nisa et al., 2006).

Three lambs were selected randomly from each group for blood collection. Ten mL

of blood was extracted from jugular vein by using syringe and transferred to vacutainer.
Serum was extracted by centrifuging at 3500 rpm. It was stored in freezer for further

analysis (Morales et al., 2010). These samples were shifted to Faisalabad in frozen form

for proximate analysis.

Laboratory Analysis

The feed and fecal samples were dried and ground to analyze DM, N and OM using
methods described by AOAC (1990)_ENREF_8 while NDF and ADF using method

described by Van Soest et al. (1991). All the chemical analysis were done in Poultry
Nutrition Laboratory at Institute of Animal Nutrition and Feed Technology, University of

Agriculture Faisalabad. Blood metabolites were analyzed in Department of Chemistry and

Biochemistry, UAF. The BUN was determined according to the method prescribed by
Berthelot (1859) and Fawcett and Scott (1960). Blood glucose was determined by using

crescent diagnostic glucose enzymatic colorimetric god-pap method (Trinder, 1969).

Statistical Analysis

The data collected for each parameter was subjected to analysis of variance using

general linear model procedure of SPSS (SPSS, 1999) and means were compared by
Duncan’s Multiple Range Test (Steel et al., 1997).

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