Page 47 - Academic Handbook FoS+29june

References:
th
1. G. Reed. Prescott and Dunn's, Industrial Microbiology, 4 Edition, McMillan Publishers, 2004.
2. A.H. Patel, Industrial Microbiology, Macmillan Publishers India Limited, 2011.
3. L.E. CasidaIndustrial Microbiology. New Age Publishers, 2016.
4. A.L. Demain, N.A. Solomon. Manual of Industrial Microbiology and Biotechnology. ASM Press, 1986.

BT3112: MICROBIOLOGICAL QUALITY CONTROL [2 1 0 3]
Microbiological Laboratory and Safe Practices: Good laboratory practices, Good microbiological practices, Biosafety cabinets –
Working of biosafety cabinets, using protective clothing, specification for BSL-1, BSL-2, BSL-3. Discarding biohazardous waste:
Methodology of Disinfection, Autoclaving & Incineration. Determining Microbes: Culture and microscopic methods - Standard
plate count, Most probable numbers, Direct microscopic counts, Biochemical and immunological methods: Limulus lysate test for
endotoxin, gel diffusion, sterility testing for pharmaceutical products, Molecular methods: Nucleic acid probes, PCR based
detection, biosensors. Pathogenic Microorganisms Enrichment culture technique, Detection of specific microorganisms - on XLD
agar, Salmonella Shigella Agar, Manitol salt agar, EMB agar, McConkey Agar, Saboraud Agar, Ascertaining microbial quality of milk
by MBRT, Rapid detection methods of microbiological quality of milk at milk collection centres (COB, 10 min Resazurin assay).
Food Safety and Microbial Standards: Hazard analysis of critical control point (HACCP) - Principles, flow diagrams, limitations,
Microbial Standards for Different Foods and Water – BIS standards for common foods and drinking water. Testing for Antibiotic
Sensitivity in Bacteria: Importance, Determination of resistance/sensitivity of bacteria using disc diffusion method, Determination
of minimal inhibitory concentration (MIC) of an antibiotic by serial double dilution method.
References:
1. W. F. Harrigan. Laboratory Methods in Food Microbiology, 3rd ed. Academic Press. 1998.
2. N. Garg, K. L. Garg and K. G. Mukerji KG. Laboratory Manual of Food Microbiology, I K International Publishing House
Pvt. Ltd. 2010.
3. J. M. Jay, M. J. Loessner, D. A. Golden, Modern Food Microbiology, 7th edition, Springer, 2005.
4. R. M. Baird, N. A. Hodges, S. P. Denyer, Handbook of Microbiological Quality control in Pharmaceutical and Medical
Devices, Taylor and Francis Inc. 2005.

BT3113: ENVIRONMENTAL MICROBIOLOGY [3 1 0 4]
Soil Microbiology: Formation and composition of soil, estimation of soil microflora, soil management, rhizosphere- positive and
negative interactions among soil microflora. Water Microbiology: Microbiology of water and water bodies, water purification,
eutrophication. Waste Water Treatment: Primary treatment, secondary treatment, advanced and final treatment. Air
Microbiology: Composition and analysis of air, aeromicroflora of different habitats, aeroallergens. Biogeochemical Cycles: Role of
microbes in Nitrogen and Carbon cycles. Water Potability: Treatment and safety of drinking (potable) water, methods to detect
potability of water samples. Role of biotechnology in pollution detection and control. Biosensors for the detection of pollutants.
Bioremediation: Use of microbes, plants, bio-surfactant and bioemulsifiers in biodegradation and biotransformation,
biodegradation of agricultural chemicals.
References:
1. R.E. Campbell. Microbial Ecology. Blackwell Scientific Publication, Oxford, England, 1983.
2. N.S. Subba Rao. Soil Microbiology. Oxford & IBH Publishing Co. New Delhi, 2017.
3. D.J. Bagyaraj and G. Rangaswamy. Agricultural Microbiology. Prentice Hall Publishers Pvt. Ltd. India, 2005.
nd
4. R.M. Maier, I.L. Pepper and C.P. Gerba. Environmental Microbiology. 2 edition, Academic Press, 2009.
nd
5. A. Martin. An Introduction to Soil Microbiology. 2 edition. John Wiley & Sons, 1977.

BT3132: MICROBIOLOGY LABORATORY-V [0 0 4 2]
To identify bacteria (E. coli, Pseudomonas) based on cultural, morphological and biochemical characteristics. Cultural
characteristics on nutrient agar and in nutrient broth, Gram characteristic, motility, presence of endospore and capsule, IMViC,
TSI, sugar fermentation, nitrate reduction, urease production, oxidase and catalase tests, To study composition of important
differential media for identification of pathogenic bacteria EMB agar, McConkey agar. To study symptoms of the diseases with the
help of photographs: Polio, anthrax, herpes, chicken pox, HPV, AIDS, dermatomycoses, Antimicrobial sensitivity testing and
determination of MIC and quality control coagulase test by Tube and Slide Method, DNAase Test, Total leukocyte and differential
leukocyte count in the given blood sample. Double diffusion by Ouchterlony method. ELISA. CVC, ESR, Platelet count. To
determine pH, moisture content, water holding capacity, percolation, capillary action of the given soil sample, Isolation of
microbes (bacteria & fungi) from
soil, Isolation of microbes (bacteria & fungi) from rhizosphere and rhizoplane, Detection (qualitative) of the presence of enzymes
(dehydrogenase, amylase, urease) in soil, Isolation of Rhizobium from root nodules of legumes, Isolation of
Azotobacter/Azospirillumfrom soil, Isolation of phosphate solubilizers from soil, determination of COD and BOD.

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