Page 47 - Хэнтий аймгийн боом өвчний голомтын судалгаа

Хэнтий аймгийн боом өвчний голомтын судалгаа

Analysis of the data on anthrax infections occurred in Khentii aimag demonstrated

that occurrences of the disease diagnosed between 1950 and 2013 were reported in

32 of 64 years. A total of 141 cases of anthrax were reported in 40 baggs of 17
soums of Khentii aimag and geographic locations of 72 foci, where carcasses of

animals dead of anthrax were destructed, were recorded.

According to our study, the animal anthrax infections occurred in the soums of
Khentii other than Tsenkhermandal soum from 1953 through 2013. As classified the

level of anthrax infected areas by A.S.Korotich et al., the outbreaks in foci of soums
of Khentii aimag are shown to be the active foci in Umnudelger and Bayankhutag

soums, the foci with periodical outbreaks in Delgerklhaan,

Jargaltkhaan,Murun,Binder, Bayan-Adraga,Batnorov, Norovlin, Bayan-Ovoo and
Batshireet soums and inactive foci Bayanmunkh, Darkhan, Galshar, Dadal and

Kherlen soums.The results of indirect ELISA testing of blood serum samples taken
from cattle grazing on the pastures near to foci by use of antigen of recombinant PA

protein as compared to the focal activities revealed that detection of specific antibody
against anthrax causative agent in 11% of serum samples of cattle in Umnudelger

and Bayankhutag soums, which are active foci, and no detection of antibody in cattle

of Dadal and Batshireet soums, which are inactive foci, allows to evaluate the level of
foci by indirect ELISA testing.

Results of bacteriological testing

The cultures in meat peptone broth forms cotton-like white precipitate, remaining the
broth transparent. The culture is brighter white color and gives R type colonies with

curled margins on meat peptone agar. Non-motile and penicillin sensitive cultures on

semi-solid medium and Gram-positive, long chain rods with spore in meat peptone
agar and broth confirm it is B.antrhacisaccording to bacteriological testing.

Results of molecular biological testing

Detection of specific bands for both 596 base pair long specific nucleotide fragment
of PA gene encoded on pXO1 plasmid and specific part of 846 base pair long

specific nucleotide fragment of capsule encoding gene located on pXO2 plasmid as

PCR testing result confirmed that the local strain is B.antrhacis, as well as the
production of capsule revealed it is a pathogenic culture/

Очирбатын Хурцбаатар 43