Page 46 - Хэнтий аймгийн боом өвчний голомтын судалгаа

Хэнтий аймгийн боом өвчний голомтын судалгаа

2.To detect the antibody on blood serum of cattle of infected areas by use of indirect

ELISA testing and determine the possibility of evaluating anthrax foci;

3.To characterize cultivability and morphology of causative agent of anthrax from
infected areas and confirm it by PCR testing.

MATERIALS AND METHODS

The data of animal anthrax diagnosed between 1950 and 2013, kept in the archive of

Veterinary department of Khentii aimag and data of human anthrax cases reported
by the National center for zoonotic disease in 1978-2013 were used. Analysis of all

reported anthrax cases was made, the coordinates of anthrax foci in the bags and
soums of Khentii aimag were determined by use of GPS equipment, geographical

map of focal points was produced by using Google map software. Focal activity for

soums of Khentii aimag was characterized according to 3 classes of anthrax infected
zone as described by A.S.Korotich. Blood samples were collected from cattle not

vaccinated against anthrax, which are kept around the anthrax foci in Umnudelger,
Bayankhutag, Batshireet, Delgerkhaan, Jargaltkhaan, Dadal, Binder and Bayan-

Adraga soums of Khentii aimag, and serum was separated from the blood samples.

Bacteriological testing

We used meat peptone broth, meat peptone agar, blood agar, nutrient medium
containing bicarbonate, semi-solid agar, Gram’s and Giemsa stain for measuring

cultivability, growth patterns and biochemical properties of bacterial isolates obtained
from animals died of anthrax in the areas of Batshireet and Bayankhutag soums of

Khentii aimag.

Molecular biological testing

DNA extracted from cultures was confirmed by PCR using the forward and reverse
primers РА5: 5’TCC TAA CAC TAA CGA AGT CG 3’ and РА8: 5’GAG GTA GAA

GGA TAT ACG GT 3’ to amplify specific fragment of gene encoding antigen located
in pXO1 plasmid and both primers BA1234: 5’CTG AGC CAT TAA TCG ATA TG 3’

and BA1301: 5’TCC CAC TTA CGT AAT CTG AG 3’ based on amplification of

specific fragment of capsule producing gene located in рХО2 plasmid.

RESULTS

Очирбатын Хурцбаатар 42