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ExtendedAbstractsoftheNinthJSME-KSMEThermalandFluidsEngineringConference October28-30，2017，Okinawa，Japan
TFEC9圃 1120
ASTUDYONDAMAGEESTIMATIONOFPLANTLEAFUNDER LOWTEMPERATURESTRESS
KYOHEIYAMASHITAペAKIRANARUMI2ANDTADASHIKONISHI3
1GraduateStudent，KanagawaInstituteofTechnology，1030Shimo-ogino，Atsugi，Kanagawa243-0292， Japan
2kanagawaInstituteofTechnologysity，1030Shimo-ogino，Atsugi，Kanagawa243-0292，Japan narumi(cù，~me.kanagawa-it.ac.jo 3OitaNationalColegeofTechnology，16Maki，Oita，Oita870-0152Japan
ABSTRACT
Cryopreservationtechniqueisveryesentialinthevariousfieldssuchasfodandmedicalenginering. Especialy，inthecaseofplant，preservationwithlivingisdesiredunderlowtemperature.However，mostof plantmaysufersomedamageduetocoldstresunderlowtemperature.Theauthorshavecontinuedto investigatethedamagedecreaseofplantunderlowtemperaれlre.Consequently，wefoundthatthemicro electriccurentloadcausesthedamagedecreaseefectonplanttisueandleaf.However，becausethereisno establishedestimationfortransientleafdamageatpresent，weightchangeratioandmicroscopicobservation betwenbeforeandaftertheexposuretolowtemper.qtureweretemporaryusedinourpreviousresearch.Then， normalized diference vegetation index (hereafter，NDVI) taking use of spectrum information in photosynthesiswasapliedinaditiontotheabovemeasures.However，NDVIwasalwaysnotasatisfactory transientmeasure.However，企omdepinspectionofthesespectrumresults，wenoticedthattherearethe characteristicchangesinwavelengthfrom500nmto630nmwiththeprogresofcoling.Onthebasisofthis spectrumcharacteristicchange，thisresearchproposedthenewmeasure(index)toestimatetransientleaf damagewithhigheracuracyanddiscusedtheposibilityofnewindex.Tounderstandleafdamage，chemical substancesrelatedtophotosynthesis;chlorophylaandscarotene，wasmeasuredunderlowtemperatureby HPLC.Consequently，itwasfoundthatnewindexwasmoresuitablethanNDVIforestimationofleafdamage.
KEYWORDS:Leaf，DamageEstimation，Lowtemperature，spectruminformation，chlorophyla，scarotene， MicroElectricCUITentLoad
1.町TRODUCTION
Cryopreservationtechniqueisveryesentialinthevariousfieldssuchasfodandmedicalenginering. However，astypicalbiomaterialcontainsalotofwater，waterspontaneouslychangestoiceoutsideorinside ofcelthroughsupercoledstatebelow企ezingpointwiththeprogresofcoling.Itisgeneralysaidthat intracelularfrezingmaycausecel'sfataldeath.Especialy，mostofplantsaredesiredtobepreservedwith living.Therefore，cryopreservationtechniqueforplantissaidtobeverydificult.Thereweremanypaperson plantdamageundercoldstres.Alabove，Yoshida'sgrouphasactivelyperformedtheinvestigationonlow temperatureaclimationofplant[1].However，thereisfewreportsfromtheviewpointsofunderstandingof intracelularfrezingbehavioursandthedamagedecreaseofplantthatweareinterestedin. Undertheabovebackground，wediscuseddamagedecreaseefectduetomicroelectriccurentloadtofind theposibilityofnewcryopreservation.Todoit，firstweconstructedthecryo-microscopicsystemequiped withahighspedcamerainaditionordinaryCCDcameraandacoledCCDcamera.Next，usingthis
*CorespondingAuthor:narumi@me.kanagawa-it.ac.jp
Copyright@ 2017byTheAuthor(s).DistributedbyJSMBandKSME，withpermision.
