Page 24 - Abstract Book ICOH4

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Gastroprotective activity of yogurt fortified with purple roselle extract in rats exposed with
2,3,7,8-TCDD
Ani Setianingrum Syafira Firdhiani , Ajeng Erika P Haskito , Ahmad Fauzi
2
1*,
3
1

1 Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya, Puncak
Dieng Eksklusif, Kalisongo, Dau, Malang, East Java, Indonesia 65151
2 Graduate student, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, East Java,
Indonesia
3 Pathology Clinic Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya, Puncak Dieng
Eksklusif, Kalisongo, Dau, Malang, East Java, Indonesia 65151

* Email corresponding author: ani.setia@ub.ac.id

ABSTRACT

2,3,7,8-Tetrachlorodiobenzo-p-dioxin (TCDD) is the most toxic dioxin compound and has many negative effects on
health. The route of TCDD into the body is by inhalation, ingestion, and skin contact. Purple Roselle is a well-known
plant in medicine. Fortified yogurt with purple rosella extract has the potential as an antioxidant. The purpose of
this study was to prove the antioxidant potential of fortified yogurt with purple rosella extracts against dioxin toxicity.
Dioxin exposure was observed on the malondialdehyde (MDA) levels and histopathology of the gastric. This study
was conducted experimentally using 25 Wistar male rats (Rattus norvegicus), which divided into five groups
consisted of a negative control group, positive control (TCDD 200 ng/kg BW), Treatment groups 1, 2, and 3 (TCDD
200 ng/kg BW + fortified yogurt with purple rosella extract concentration 0,5%, concentration 1%, and
concentration 1,5%) given orally. MDA level was measured by Thiobarbituric Acid (TBA) method quantitatively using
the one-way Analysis of Variance (ANOVA) statistical test followed by Tukey post hoc test with 95% confidence
interval. The histopathology of the gastric was stained by the Hematoxylin-eosin staining method, which is observed
descriptively. The result of this study showed that fortified yogurt with purple rosella extract 1% could prevent the
increased level of gastric MDA level significantly (p<0,05) and repair histopathological damage to the lamina
propria mucosa of the gastric rat exposed to TCDD. The conclusion of this study was the administration of yogurt
with purple rosella extract could prevent the increased level of MDA and repair histopathological damage to the
gastric exposed to TCDD.
Keywords: dioxins, gastric, histopathology, MDA, yogurt, purple roselle

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