Page 26 - Abstract Book ICOH4

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Evaluation Of DNA Isolation and Amplified cGH Through Frozen, Formalin-

Fixed, and Paraffin-Embedded Tissue Sample

Mifta Rizqina Amalia 1,* , Yudit Oktanella 2, Andreas Bandang Hardian 3, Dini Agusti Pramanandi 4, Fajar
Shodiq Permata 5, Kharisma Kurnia Utami 6, Andi Tri Rakhmat Akbar 7, Made Venika Nareswari 8

1Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Kec. Dau,
Kab. Malang 65151, Indonesia
*Correspondence to: miftaramalia@gmail.com, +62813-4773-2299

Abstract

Optimization of sample storage is needed to prevent damage to DNA quality. The current tissue storage method
commonly used is the fresh frozen sample in -20 oC to -196 oC. Formalin induces DNA crosslink presence.
Meanwhile, paraffin-embedded tissue is used as a histology-based method by infiltrating and maintaining the
cell structure with paraffin. It is necessary to choose an appropriate tissue sample preservation method related
to the materials and tools availability. The purpose of this study was to compare the purity, concentration, and
DNA band visualization of the isolated sample and PCR amplicon from each storage method. Tissue samples
were stored at frozen temperature -20 oC, 10% NS formalin, and paraffin-embedded preparations. The 260/230
and 260/280 absorbance values and electrophoresis of 0.8% and 2% agarose gel visualization were analyzed.
The result showed no significant differences among all of the methods in DNA purity and concentration. Frozen
sample isolates and PCR amplicon visualized a good DNA band. Paraffinized tissue's PCR amplicon visualized
thin DNA band, and either formalin-fixed tissue isolates or its PCR amplicon showed no DNA band. In conclusion,
all the tissue storage methods can be applied for DNA isolation and PCR examination where all storage methods
showed a good purity and concentration quantitively, while the formalin-fixed and paraffinized tissue storage
method showed a slightly lower DNA in quality.

Keywords: DNA isolation, Electrophoresis, Formalin-fixed, Paraffin-embedded, PCR

THE 4 INTERNATIONAL CONFERENCE ON ONE HEALTH (ICOH)
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“Strengthening Collaboration in One Health Systems”