Page 10 - PowerPoint 簡報
P. 10
EpiTYPER DNA Methylation analysis
CpG CpG CpG CpG CpG CpG
Bisulfite BS/PCR
Treatment
CpG UpG UpG CpG CpG CpG
T7 IVT dC, rU, rG, rA
PCR
Amplification GpC ApC ApC GpC GpC GpC
RNase A base specific cleavage (rU)
In vitro GpC ApC ApC GpC GpC GpC
transcription
ApC ApC
ApC ApC ApC ApC GpC ApC
GpC GpC GpC GpC
Base specific GpC GpC
cleavage
AC GC
Mass Detection
3000Da
7000Da
and Ratio Unmethylated Methylated 4500Da 6000Da 10
Analysis
1500Da
