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LAB ACTIVITIES

3. LIGHT IS ESSENTIAL FOR PHOTOSYNTHESIS 4. O IS LIBERATED DURING PHOTOSYNTHESIS
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AIM original preparation AIM
To prove that light is essential for photysynthesis. lab activity To prove that O is liberates during photosynthesis.
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APPARATUS APPARATUS
1. Beaker 2. Test tube 3. Hydrilla plants
1. potted plant 3. Piece of black chart
2. Iodine solution 4. Clips 4. Glass funnel 5. Water 6. Glowing splinter
RAJU’S NATURAL SCIENCE ACADEMY
DIAGRAM DIAGRAM

PROCEDURE
1. Take a beaker.
4. Insert hydralla plants in it.
3. Take a funnel. 2. Fill with water.
PROCEDURE 5. Keep funnel into the beaker as shown in the diagram.
1. Take a potted plant. 6. Take a test tube filled with water. 7. Invert it over the funnel.
2. Keep it into the darkroom for a week days. 8. Keep the entire setup in sunlight.
(due to this, leaves are free from starch) OBSERVATION
3. Select a leaf and cover it with a piece of black chart as shown in the diagram. 1. Small gas bubbles are come out from the hydrilla plants.
(black chart stops sunlight. So, that part of leaf does not exposed to sun light) 2. These gas bubbles collected at the end of the test tube.
4. Keep the entire set up in sunlight for 4-5 hours. 3. Then test the gas with splinter.
5. Detach the leaf from the plant. 4. The splinter glows and burns vigorously.
6. Remove the chart piece and test the leaf by using iodine solution. INFERENCE
OBSERVATION
Entire leaf turns blue-black colour except the part covered by the black chart. This experiment proves that O is liberated during photosynthesis.
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INFERENCE PRECAUTIONS
This experiment proves that light is essential for photosynthesis. 1. We should use sub merged aquatic plants only.
PRECAUTIONS 2. Carefully invert and remove the test tube without entering air.
We should fix the black chart on the leaf tightly by using clips.

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