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LAB ACTIVITIES

7. ANAEROBIC RESPIRATION IN YEAST 8. DEMONSTRATION OF ROOT PRESSURE
AIM AIM
To understand anaerobic respiration. To demonstrate root pressure in plant.
APPARATUS
original preparation APPARATUS
1. Thermos flask 4. Thermometer 7. Paraffin lab activity 1. Potted plant 2. Rubber tube 3. Glass tube
pl. scan QR
2. Yeast 5. Two holed rubber corks - 2 8. Glass tubes - 2 4. Clamp 5. Water 6. Cutter
3. Glucose solution 6. Wash bottle 9. Lime water
RAJU’S NATURAL SCIENCE ACADEMY
DIAGRAM DIAGRAM

PROCEDURE
1. Take a glucose solution into the thermos flask.
2. Boil it for a minute. (It removes dissolced oxygen O from glucose solution) PROCEDURE
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3. Then cooling it without shaking. 4. Add some yeast to the glucose solution. 1. Take a potted plant.
5. Pour 1 cm layer of liquid paraffin on the glucose solution. 2. Cut the stem 1cm above the ground level.
6. Close thermos flask with two holed rubber cork. 3. Connect a glass tube with the help of a rubber tube as shown in the diagram.
7. Arrange thermometer, glass tubes, wash bottle with lime water shown in the diagram. 4. Pour water in the glass tube.
8. Keep the arrangement undisturbed for two days. 5. Mark the level of water in glass tube.
OBSERVATION
1. Lime water turn into milky white. 6. Leave arrangement for 2-3 hours.
(CO is liberated from yeast during anaerobic respiration) 7. Observe the water level in the tube.
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2. The temperature slowly increased in the thermometer. OBSERVATION
(heat is liberated from yeast during anaerobic respiration)
3. Alcohol smell comes out from the flask Water level increased due to root pressure.
(alcohol is liberated from yeast during anaerobic respiration) INFERENCE
INFERENCE : Through this experiment we understood anaerobic respiration. This experiment shows root pressure in plant.
PRECAUTIONS 1. The bulb of the thermometer should dip in the glucose solution.
2. Close the flask with tight fitting cork. PRECAUTIONS
3. First end of the glass tube does not dipped in the glucose solution, 1. The size of glass tube should be equal to the size of the stem.
but second end must dip in the lime water. 2. Join the tube and stem tightly.
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