Page 31 - Feed Lab

Reagents and Chemicals Suguna Management System Ver 1.0 / SOP / FQL / P1 - 04 Page 3 of 6

If the substance is liquid, (1+4) H SO - 100 ml of H SO in 400ml of water.
4
2
4
2
Volume x Density = Weight
Preparation of some common indicators
Volume = Weight /Density FMethyl red: 0.5% solution of the substance in
ethanol or methanol
= X gm / Density
FMethyl orange: 0.05% solution of AR grade
= Y ml /L
chemical in Ethanol.

FPhenolphthalein: 1% solution in methanol or
Preparation of (X + Y) Acid solution
X ml of acid in Y ml of water ethanol
For eg. : FEriochrome Black: 5% solution of the AR grade in
ethanol.
(1+3) HCl - 100 ml of HCl in 300 ml of water.

Preparation of some common standard solutions

Chemical (AR Grade) Weight in 1lit of H2O
Standardization With Indicator
0.2N EDTA 3.72 g of EDTA CaCO 3 Erichrome Black T
FeSO4.7H 0 7.8g of FeSO4.7H 0 K Cr O 7 Diphenylamine
2
2
2
2
HCl 9ml Concentrated HCl NaOH Phenolphthalein
HNO 3 6.3 ml of Con HNO 3 NaOH Phenolphthalein
H SO 4 2.78 ml of Con H SO 4 Na CO 3 Methyl Red
2
2
2
2
H C O 4 6.303gm of H C O 4 NaOH Phenolphthalein
2
2
2
KmnO 4 3.161 g of KmnO 4 H C O 4 Self indicator
2
2
AgNO 3 16.987 g of AgNO 3 NaCl K CrO 4
2
NaOH 4gm of NaOH H SO 4 Phenolphthalein
2
Equivalent weight of some common chemicals:
Equivalent Weight Equivalent Weight
Chemicals Chemicals
169.89 63.00
AgNO 3 HNO 3
CaCO 3 50.04 KMnO 4 31.60

EDTA 186.10 K Cr O7 49.03
2
2
FeSO .7H O 278.00 NaOH 40.00
4
2
(NH ) Fe(SO ) .6H O 392.13 NaCl 58.45
4 2
4
2
2
HCl 36.46 H C O .2H O 63.03
2
4
2
2
H SO 49.04 Na CO 53.00
2 4 2 3
Prepared by : Approved by :
21 Director - Technical