Page 6 - Equine influenza e-Book

Serology

• Detect antibody detection either with a hemagglutination inhibition (HI) or single radial hemolysis (SRH)
• SRH is more repeatable between laboratories and can detect smaller increases in antibody response but
HI is less labor intensive and thus more commonly used.
• In unvaccinated horses a H3 titer indicates exposure.
• In vaccinated horses a H3 titer greater than H7 titer indicates exposure.
• ELISA for antibody against nuceloprotein; can help distinguish vaccinated from infected horses.

2-D Ultrasonography

• Cranioventral abnormalities about 7 days following infection.
• Resolution of changes by 14 days following infection.

Hematology

• White blood cell count Blood: leukocytes Blood: lymphocytes:
o Initially may decrease.
o At 14-21 days increase: 10-12 x 10*9/l.
• Anemia Blood: hemoglobin (Hb) concentration.

Confirmation of diagnosis

Discriminatory diagnostic features

• Serology.
• Clinical signs.
• History.
• Hematology.

Definitive diagnostic features

• Virology.

Gross autopsy findings

• Bronchiolitis (serous to mucoid or mucopurulent).
• Secondary bacterial bronchopneumonia.

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