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Centromere pattern (AC-3)

Hep-2 cells, which is characterised by fine, evenly sized granule

(generally 46 or 92 centromere per cell nucleus).

The granules in interphase cells are spread evenly over

the nucleus, while in mitotic cells they are arranged either ribbon-

like on the equatorial plane (metaphase) or in two parallel ribbons

approaching the centrioles (anaphase).

Primate liver, Which are spread over the cell nucleus, the

fluorsecence of these granules is significantly weaker than Hep-2

cell staining and is therefore easy to miss. Mitotic cells are only

rarely detected on liver substrate.