Page 6 - Technical Saw Palmetto Booklet
P. 6
MTC Industries, Inc.
Herbsine Bioengineering Co., Ltd.
2. HPTLC
1 2 3 4 5 6 7 8 9
Figure 4 HPTLC chromatography of various Oils
Lane 1 Saw Palmetto oil, UV-171023
Lane 2 Saw Palmetto oil, EE-1800628
Lane 3 Saw Palmetto oil, SN-180401
Lane 4 Saw Palmetto Berry, Origin: Florida, American
Lane 5 Olive oil
Lane 6 Pumpkin seed oil
Lane 7 Flax seed oil
Lane 8 Coconut oil
Lane 9 Soybean oil
Prep. 1.0 g+100 mL EtOH Sonicate/heat@~50℃~1/2 hr
Silica gel 60, F254 RP-18, 10×10 cm HPTLC plate
Stationary Phase
[Merck Part#:1.13724.0001, LOT#: HX67519324]
Mobile Phase Dichloromethane: acetic acid: acetone = 2: 4: 5, V / V / V
Detection 2.5% Phosphomolybdic acid reagent →110℃ 5min →Daylight
Sample origin @ 15mm, Solvent front @ 90mm
Saw palmetto oil has an obvious characteristic chromatography that can be easily
distinguished from other vegetable oils by HPTLC.
255 Oser Ave., Hauppauge, NY 11788 Tel: 631-274-4818 4/28
