Page 116 - Live-cellanalysis handbook

Live-Cell Analysis Handbook — Third Edition

A iCell GlutaNeurons B iCell GABANeurons

Mean Active Mean Active
correlation objects correlation objects
1.0 2000 1.0 1000
Correlation
Active objects
0.8 0.8 800
1500

0.6 0.6 600
Active objects 1000
0.4 0.4 400

500
0.2 0.2 200
Correlation
0.0 0 0.0 0
0 5 10 15 20 25 30 0 5 10 15 20 25 30
Days Days

C iCell DopaNeurons D CNS.4U
Mean Active Mean Active
correlation objects correlation objects
1.0 3000 1.0 1500
Active objects Active objects
0.8 0.8

2000 1000
0.6 Correlation 0.6

0.4 0.4
1000 500

0.2 0.2
Correlation
0.0 0 0.0 0
0 5 10 15 20 25 30 0 5 10 15 20 25 30
Days Days
Figure 4. Comparison of differences in functional activity in iPSC-derived neurons. iCell GlutaNeurons, iCell GABANeurons, iCell DopaNeurons (Cellular Dynamics
International) and CNS.4U neurons (Ncardia) were all seeded at 20K cells/well. iCell GlutaNeurons, iCell GABANeurons and iCell DopaNeurons were also plated
with a co-culture of rat astrocytes seeded at 15K cells/well. Neurons were infected with NeuroBurst Orange reagent, and active object number and mean
correlation were quantified for up to 45 days. Example calcium oscillation traces and kinetic graphs of activity metrics over time for iCell GlutaNeurons (A) and
iCell GABANeurons (B). Mean correlation and active object number were quantified for iCell DopaNeurons (25 days) (C) and CNS.4U neurons (45 days) (D). Data
points represent Mean ± SEM.

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