Page 121 - Live-cellanalysis handbook

Kinetic Neuroimmune Assays

IncuCyte neuroimmune assays at a glance

In order to measure microglia function over time, various the chemotactic response is generated throughout the experiment.
quantitative and qualitative assays are employed. IncuCyte In addition, visual assessment of morphological changes associated
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HD phase imaging allows for visual observation of activation- with stimuli response as the membrane of the plate is optically
associated morphology changes, giving detailed insight into clear.
cellular changes associated with neurodegenerative diseases.
IncuCyte Phagocytosis Assays enable real-time, automated
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IncuCyte Chemotaxis Assays are fully automated solutions to analysis of both phagocytosis and efferocytosis within your cell
quantify directional migration in response to a stimulus. Utilizing a culture incubator. Using non-perturbing, pH sensitive fluorescent
proprietary migration microplate, the IncuCyte ClearView 96-Well probes, visualization and quantification of phagocytosis can be
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Chemotaxis Plate, label-free analysis and precise quantification of achieved in real-time over the entire assay time course.

Shortcomings of Traditional Assays Live-Cell Imaging and Analysis Approaches

• Requires fixation and immunostaining steps. • Real-time, label-free measurement of chemotaxis migration
without fixing, staining or cell scraping steps.
• Measurements at user-defined time points. • Real-time automated analysis in 96- or 384-well formats.
• Inability to visualize, confirm morphological changes. • Direct visualization of morphological changes associated with
biological response.
• Requirement for large number of cells for single-point • Analyze sensitive and rare cells with a cell sparing, highly
measurements. reproducible 96- or 384-well assays.

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