Page 99 - Live-cellanalysis handbook

Kinetic Single Spheroid Assays

Conclusions

3D micro-tissues and organoids are becoming increasingly • Miniaturization of the assay to provide a high-throughput
popular for their more relevant morphology and cell survival, but method of producing reproducible, quantitative
current methods for assessing the growth and vitality of these pharmacological data in physiologically relevant cell models
models are limited. Most assays require cell labeling and only simultaneously for a variety of test compound.
provide a single time point readout. By contrast, the IncuCyte® • Flexibility in cell types and co-culture, enabling extension of
S3 Live-Cell Assay allows: the assay for study of immune modulators.

• Label-free study of spheroid morphology, growth and shrinkage • IncuCyte® S3 Live-Cell Single Spheroid Assay provides a
in 96- and 384-well format. valuable and timely addition to the toolkit of those working
in basic biology, medical research, and pharmacological drug
• Generation of kinetic measurements and cell-dependent growth development who seek to better understand cellular responses.
curves for spheroids derived from different types of cells.
• Monitoring of spheroid viability over time and in different
treatment conditions using IncuCyte’s Cell Health Reagents and/
or stable expression of fluorescent reporter proteins and the
ability to link this to morphological observations.

97