Page 102 - Live-cellanalysis handbook

Live-Cell Analysis Handbook — Third Edition

How Live-Cell Assays for Neuroscience Work

®
IncuCyte Neurite Analysis Assays active cell within each well to reveal the extent of connections in
a network and automatically quantifies longitudinal changes of
In the neurite analysis assay, image software analysis, IncuCyte® activity for the characterization of neuronal cell models.
NeuroTrack Analysis Software module, is employed for automated
quantification of neurite dynamics. This assay permits the analysis Kinetic Neuroimmune Assays
of neurons in monoculture (label-free) or in co-culture with
astrocytes, using a non-perturbing IncuCyte® NeuroLight Orange To quantify neuroimmune function, applications include
Lentivirus reagent for continuous analysis of neurite length and phagocytosis assays using non-perturbing cell-labeling reagents
branch points. Furthermore, the neurite analysis assay can be and chemotactic migration assays utilizing purpose-built
multiplexed with cell health reagents, IncuCyte® AnnexinV NIR or consumables and software. The IncuCyte® pHrodo® Orange Cell
Orange, to determine the onset of apoptosis in real time. labeling kit utilizes a pH-sensitive fluorescent probe to quantify
cell clearance of diseased or dying cells or neuronal-associated
Kinetic Neuronal Activity Assay proteins to efficiently study the full time course of phagocytosis in
the model of your choice.
To detect changes in neuronal activity, an end-to-end solution
consisting of instrumentation, software and reagent is employed. To evaluate microglial response, the IncuCyte® Chemotaxis
Addition of a genetically-encoded calcium indicator, IncuCyte® Cell Migration Assay enables real-time visualization and
NeuroBurst Orange Lentiviral Reagent, allows for efficient, non- quantification of cell migration in response to a chemical
perturbing labeling of living neurons for the long-term detection stimulus. Using an optically clear membrane, visual assessments
of neuronal activity via calcium binding. Integrated software of morphology can be linked to real-time measurements to gain
captures and analyzes short-term calcium flux kinetics for every deep phenotypic insights.

References

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