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1161.e2 Semen Collection, Cat

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SEMEN COLLECTION, CAT Chemical ejaculation with urethral catheterization in SEMEN COLLECTION, CAT Electroejaculation in a tomcat. Tomcats should
a tomcat. Tomcats are intramuscularly injected with medetomidine (130-140 mcg/kg be anesthetized with dexmedetomidine (30-40 mcg/kg) and ketamine (3-5 mg/kg)
of body weight). After the pharmacologic effect is obtained, a modified, open-ended, injected intramuscularly. Electroejaculation is performed using a rectal probe 1 cm
3.5-Fr tomcat catheter is passed 9 cm into the urethra and then simply removed. in diameter and 12-13 cm long. The rectal probe is lubricated with nonspermicidal
After the catheter is removed, attach a syringe that already has air drawn up into lubricant and inserted gently into the rectum approximately 5-7 cm, pointing the
it to allow the sample to be forced out into a microcentrifuge tube for further electrodes ventrally. The penis can be manually extended and cleaned with gauze
processing. (Courtesy Dr. Daniele Zambelli.) moistened with saline and then dried. A sterile Eppendorf tube is placed at the
tip of the penis without allowing the tip of the penis to touch the tube. A series
of electrical stimuli are administered by the electroejaculator by turning the dial
to the desired voltage for 2-3 seconds and then abruptly back to zero for 2-3
seconds. (Courtesy Dr. Daniele Zambelli.)
• A new, sterile tube should be used between
each set after collection to prevent contami-
nation or loss of the sample. • Keep all equipment used in the collection Zambelli D, et al: Collection of semen by urethral
• Modified, 3.5-Fr tomcat catheter of feline semen at 37°C. catheterization after pharmacologically induced
○ Most tomcat catheters are 11 cm long. • Have anesthetic reversal pulled up in a syringe spermatozoa releasing in the domestic cat. In
○ Cut the end with a sharp blade (scalpel) before procedure in case of emergency Proceedings of the 5th Biannual Congress of the
to remove lateral openings and to obtain European Veterinary Society for Small Animal
a shorter, open-ended catheter. SUGGESTED READINGS Reproduction (EVSSAR), p 300, 2006, Budapest,
○ Make sure the end is patent but also not Hungary.
sharp enough to cause any harm to the Filliers M, et al: In vitro evaluation of fresh sperm AUTHOR: Clare M. Scully, DVM, MA, MS, DACT
quality in tomcats: a comparison of two collection
urethra. techniques. Theriogenology 74(1):31-39, 2010. EDITORS: Leah A. Cohn, DVM, PhD, DACVIM;
○ It is measured 9 cm from the end that will Johnson AK, et al: Semen collection, evaluation, Michelle A. Kutzler, DVM, PhD, DACT
be inserted into the urethra and carefully and cryopreservation in the domestic feline. Clin
marked with a Sharpie. Theriogenol 2(3):233-238, 2010.

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