Page 624 - Small Animal Internal Medicine, 6th Edition
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596    PART IV   Hepatobiliary and Exocrine Pancreatic Disorders


            hepatitis. These dogs usually present at about 4 years of age,   Diagnosis
            but may be younger  (Fig. 36.6).  Eventually, if  not treated,   The magnitude of increase in liver enzyme activities and the
  VetBooks.ir  affected dogs will develop cirrhosis.             storage disease are very similar to those of dogs with idio-
                                                                 diagnostic imaging findings in dogs with chronic copper
              The clinical signs and progression in other breeds with
                                                                 pathic chronic hepatitis. Therefore a definitive diagnosis
            copper storage disease are similar to those in Bedlington
            Terriers. The disease in Dalmatians is associated with acute   requires a liver biopsy and determination or estimation
            onset, rapid progression, and very high levels of hepatic   of the copper concentration in the liver. This can be done
            copper in the absence of significant clinical, clinicopatho-  qualitatively on formalin-fixed sections using rhodanine or
            logic, or histologic evidence of cholestasis. Affected dogs   rubeanic acid staining to detect copper; correlations between
            usually present as young adults with acute onset of GI signs   the quantitative and qualitative estimations of copper accu-
            and  PU-PD,  by  which  time  severe  liver  disease  is  already   mulation have been published (Shih et al., 2007). Digital
            present.  Labrador  Retrievers with  copper  storage disease   scanning of rhodanine-stained sections is a more objective
            have an average age at presentation of 7 to 9 years (range,   qualitative measure of hepatic copper (Center et al., 2013).
            2.5–14 years). The clinical signs are relatively mild and   The finding of large accumulations of copper in hepatocytes
            include anorexia, vomiting, and lethargy. Doberman Pin-  on cytology with rubeanic acid is also suggestive of copper
            schers appear to have a long phase of subclinical disease   storage disease (Fig. 36.7;  Teske et al., 1992). Quantitative
            culminating, in untreated cases, in an acute-on-chronic   measurement of copper content can also be performed, but
            disease and rapidly progressive deterioration. However, it is   this requires a large biopsy specimen carefully taken and
            unclear how many of the clinically affected Doberman Pin-  stored in copper-free tubes. The concentration of copper that
            schers described in the literature had copper storage disease   is considered pathologic is controversial but > 1000 µg/g dry
            and how many had idiopathic or potentially immune-   weight liver is generally considered significant. The clinician
            mediated chronic hepatitis, so the actual presenting signs of   should also be aware that there can be considerable variation
            copper storage disease in this breed are unclear. Most pub-  in copper concentration between liver lobes due to patchy
            lished studies on copper storage disease in Doberman Pin-  fibrosis and regeneration. Further details on diagnosis of
            schers have described the diagnosis and treatment of   copper storage disease can be found in Webster et al., 2019.
            subclinical disease.                                 In addition to estimating copper content, the liver biopsy
              Occasionally, dogs with copper storage disease also   will give an indication of the chronicity and extent of liver
            present with renal signs typical of Fanconi syndrome with   damage, which will affect treatment decisions similarly to
            normoglycemic glycosuria and proteinuria. This has not   that for chronic hepatitis. Bedlington Terriers can be tested
            been reported in Bedlington Terriers but in a variety of   for the COMMD1 deletion before breeding or when newly
            breeds including two West Highland White Terriers (Apple-  acquired to assess their risk for this disease, but an absence
            man et al., 2008; Hill et al., 2008) and nine Labrador Retriev-  of the COMMD1 deletion does not guarantee that the dog
            ers (Langlois et al., 2013). Most but not all cases have been
            associated with increased copper  accumulation  in the
            kidneys on histology.
























                                                                 FIG 36.7
                                                                 Cytology of hepatocytes from Bedlington Terrier with copper
            FIG 36.6                                             storage disease demonstrating copper granules (rubeanic
            Bedlington Terrier with copper storage disease. (From Hall   acid stain). (Courtesy Elizabeth Villiers; from Hall EJ et al.,
            EJ et al., editors: BSAVA manual of canine and feline   editors: BSAVA manual of canine and feline
            gastroenterology, ed 2, Gloucestershire, United Kingdom,   gastroenterology, ed 2, Gloucestershire, United Kingdom,
            2005, British Small Animal Veterinary Association.)  2005, British Small Animal Veterinary Association.)
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