Page 354 - The Veterinary Laboratory and Field Manual 3rd Edition

Serology and immunology 323

(a)

(b)

Figure 6.13 (a) ELISA technique for measuring the amount of antigen in a test sample (indirect method).
(b) ELISA technique for measuring the amount of antibody in a test serum (indirect method). (A) Antigen
coated to plate; (B) antibody binds to antigen, the plate is washed; (C) anti-species antibody bound to enzyme
is added; (D) after washing the plate, substrate is added which reacts with bound antibody-enzyme complex.
A colour reaction can then be measured. Source: M. Sarjoon Abdul-Cader, University of Calgary, Canada.

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