Page 246 - Natural Antioxidants, Applications in Foods of Animal Origin

Methods and Their Applications for Measuring 225
VetBooks.ir Two methods are used for analyzing 2-TBARSs in meat products that

are widely used in the meat industry (Fig. 6.16). The distillation method
involves partitioning secondary oxidation products in the meat sample into
the appropriate solvent then separating these compounds by distillation.
The direct method determines the concentration of secondary oxidation
products directly without partitioning the secondary oxidation products or

Methods for QuantifYing 2- Thiobarbituric Acid Reactive Substances in Meats
Generalize Flow Diagram
TBARS
I I Distillation Methods I

with
TBARS for Beef TBARS for Poultry
I I I
Transfer an aliquot of lh e test I TBARS for Pork I
so ln. into test tube conta ining
the recommended quant ity of
TBA reagent. I Weigh sample add I :3 w/v in
trichloroacetic acid and
homogenize.
,. +
I Vacuum filter homogenate
through Watman #42 ash less
filter paper.
t
Cool, pipette sample into
cuvette, read absorbance at I Add TBA reagent incubate in
530nm from calibrated boiling for 40 min ..
spectrophotometer with b and t
width < 5nm. Determine cone,
using Beer Lambert Law Conduct "spike and !
recovery" with a
small aliquot of
Pipette an aliquot or standardized soln. of Cool, then pipette into cuvette,
distillate from meat MDA-bis acetaL read absorbance at 530 nm in
homogenate into test calibrated spectrophotomer,
tube with TBA band width < 5nm. Calculate
rea ~ent. cone. Using Beer Lambert Law

~
I Pipette an aliquot of distillate from meat
homogenate into test tube with TBA reagent. I
~ ~

Incubate in boiling water for 35 min., cool then pipette
sample into cuvette. Read absorbance at 530nm from
calibrated spectrophotometer with spectral band width
< Snm. Determine cone, use Beer Lambert Law.

FIGURE 6.16 Methods and techniques for the analysis of TBARS (thiobarbituric acid
reactive substances).