Page 208 - Essential Haematology

194 / Chapter 14 Chronic myeloid leukaemia

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46,XY,t(9;22)(q34;q11)
(d)

(i) (ii)
(e)
Figure 14.1 (Continued) (d) Karyotype showing the t(9; 22) (q34; q11) translocation. The Ph chromosome is
arrowed. (e) Visualization of the Philadelphia chromosome on: (i) dividing (metaphase); and (ii) quiescent
(interphase) cells by fl uorescence in situ hybridization (FISH) analysis (ABL probe in red and BCR probe in
green) with fusion signals (red/green) on the Ph and der(9) chromosomes. (Courtesy of Dr Ellie Nacheva)