Page 45 - IPCoSME 2021

1 INTERNATIONAL POSTGRADUATE CONFERENCE ON SCIENCE AND MARINE ENVIRONMENT 2021
st
(IPCoSME 2021)
“Environmental Sustainability Enhancement Through the Collaboration of Sciences”

RE-03

A CO-CULTURE BETWEEN FUSARIUM SP. AND STREPTOMYCES SP. SUK10
USING METABOLOMIC APPROACH AND ITS BIOLOGICAL ACTIVITIES

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MUHAMMAD ASYRAF ZAWAWI , MARIAM TAIB , KAMARIAH BAKAR ,
NORAZIAH MOHAMAD ZIN , SAIF ALDEEN MOHAMMAD FAYIZ JABER ,
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RUANGELIE EDRADA-EBEL AND NOOR WINI MAZLAN 1,2*
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1 Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala
Nerus, Terengganu, Malaysia
2 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Nerus,
Terengganu, Malaysia
3 Center of Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences,
Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, 50300 Kuala Lumpur, Malaysia
4 Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The
John Arbuthnott Building, 161 Cathedral Street, Glasgow G4 0RE, Scotland

*Corresponding author email: noorwini@umt.edu.my

Abstract: Microorganisms including fungi and bacteria play important roles in natural product

drug discovery to treat various diseases. Due to misuse of available drugs, antimicrobial
resistance (AMR) of bacteria was developed. Besides, isolation and identification of

metabolites use classical approach often lead to re-discovery of known metabolites, perhaps
largely unexplored. Various experimental designs have been attempted to produce new

metabolites with different and enhance biological activities. Thus, in this study, a co-culture

method is used to activate silent gene clusters of microbial to suppress the capability of
microbial in producing new secondary metabolites, which are usually silent under individual

growth condition. The co-culture between Fusarium sp. and Streptomyces sp. SUK10 used
different media and growth condition to give crude extracts. Only the crude extract from co-

culture day 7 in Wickerham broth (FS7) showed significant antibacterial activity on the Gram-
positive bacteria- Micrococcus sp., Staphylococcus aureus and Bacillus cereus with minimum

inhibition concentration (MIC) values of 1.25 mg/mL, 0.313 mg/mL, and 1.25 mg/mL,

respectively, compared with mono- and other co-culture extracts were inactive. Meanwhile,
nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry

coupled with multivariate analysis and dereplication study were used to fast track the

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