Page 46 - IPCoSME 2021

1 INTERNATIONAL POSTGRADUATE CONFERENCE ON SCIENCE AND MARINE ENVIRONMENT 2021
st
(IPCoSME 2021)
“Environmental Sustainability Enhancement Through the Collaboration of Sciences”

identification of metabolites produced in different culture method. The differences in chemical

profiles between mono- and co-cultures extracts were confirmed using principal component
analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS-

DA). The loadings plot of unsupervised PCA and supervised OPLS-DA analysis showed that

the co-culture FS7 extract was outlier, giving the known metabolites identified as 4-hydroxy-
2-nonylquinoline (1), 3-hydroxydecanoic acid (2) and FA 4283 (3) (Figure 1), while the

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-
+
unknown metabolites with m/z 649.382 [M-H] , 677.413 [M-H] , 527.320 [M+H] and
+
1031.650 [M+H] which were absence in their parent cultures. In conclusion, the co-culture
triggers the production of potent new metabolites which could enhance the antibacterial
activity.

Keywords: Fusarium sp., Streptomyces sp., co-culture, multivariate, metabolomics

1 2 3

Figure 1. Secondary metabolites 1-3 which were produced in the co-culture between

Fusarium sp. and Streptomyces sp. SUK10, day 7 (FS7).

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