Page 49 - IPCoSME 2021

1 INTERNATIONAL POSTGRADUATE CONFERENCE ON SCIENCE AND MARINE ENVIRONMENT 2021
st
(IPCoSME 2021)
“Environmental Sustainability Enhancement Through the Collaboration of Sciences”

RE-05

ELUCIDATION OF THE SECONDARY METABOLITES FROM A CO-CULTURE
BETWEEN Fusarium sp. AND Curvularia sp. AND THEIR BIOLOGICAL

ACTIVITIES

1
1
NURUL IZZATI ROSDI , KHOR CHEN TER AND NOOR WINI MAZLAN 1,2*

1 Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala
Nerus, Terengganu, Malaysia
2 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Kuala Nerus,
Terengganu, Malaysia

*Corresponding author email: noorwini@umt.edu.my

Abstract: Endophytic fungi are important in drug discovery in searching novel and active

metabolites due to its sustainable and re-producible under laboratory compared with the host
plants. However, the use of single culture usually leads to the production of known compounds

with similar bioactivities. Therefore, in this study, a co-culture method between endophytic
fungi Curvularia sp. and Fusarium sp. which have been isolated from mangrove plant

Avicennia lanata was performed in rice medium at different growth stages to trigger the

production of different metabolites which are not in their parent cultures. Evaluation of
antioxidant activity on the crude extracts used DPPH scavenging assay, possessing in enhance

activity on the crude extract from co-culture on day 15 (FC15) with 50% inhibition
concentration (IC50) value of 2.8 mg/mL compared with mono- and other co-culture extracts.

Meanwhile, fractionation of the FC15 extract gave 17 major fractions, in which fraction F17

showed potent antibacterial activity on the Gram-positive bacteria Micrococcus sp. and
Bacillus cereus with minimum inhibition concentration values of 0.3125 mg/mL and 5 mg/mL,

respectively. Further isolation on FC15 extract gave three pure compounds and their molecule
structures were identified using one and two dimensional nuclear magnetic resonance

spectroscopy, namely as monocerin (1), ergosterol peroxide (2) and acuminatopyrone (3)
(Figure 1). The cytotoxicity activity on the isolated compounds were also investigated using

MTT assay, in which compounds 1-3 showed potent activity on cervical cancer (HeLa) cell

with IC50 values of 1.531 µg/mL, 6.848 µg/mL and 10.34 µg/mL, respectively. While the
cytotoxicity on breast cancer (MCF-7) cell of compounds 1-3 showed significant activity with

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