Page 45 - Phytochemistry 2 (06-PG 605)

Estimation of d- tubocurarine chloride
 Tubocurarine can be quantitatively estimated either biologically or spectro-

photometrically.
1- Biological assay (head-drop method)
It is the smallest amount of the drug capable of producing a full head drop in
50% or more of
the test group.
2- Spectrophotometric assay:
Based on measuring the absorbance at λmax 280 nm.
Uses
Skeletal muscle relaxant.
- Used as adjuvant to anaesthesia to secure muscular relaxation in surgical
operations,
- Control and reduce convulsions of strychnine poisoning and tetanus

Opium alkaloids

• Opium is the air dried latex (of milky exudate) obtained by the incision from
the unripe capsule of Papaver somniferum (Papaveraceae).

• It contains not less than 10% of morphine and not less than 2% of codeine.

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