Page 296 - Applied Pharmacognosy

Types of Chemical markers

 As the protein migrates through the pH gradient, its
net charge and mobility will decrease and the
protein will slow down.

 Eventually, the protein will arrive at the point in
the pH gradient equaling its pI. There, being
uncharged, it will stop migrating, and focus into
sharp band.
 In this way, different proteins focus into sharp
bands in the pH gradient at their individual,
characteristic pI values.