Page 496 - Applied Pharmacognosy

• most cellular constituents (cellulose, proteins, amino acids,
starch, fats) are sparingly soluble and are thus removed.

•

• The water/acetonitrile sample contains the pesticide to
be evaluated and some polar constituents.

• The water/acetonitrile sample is extracted with light
petroleum.

• light petroleum fraction contains the pesticide

• purification is required, using silica gel column
chromatography eluting with a mixture of light
petroleum and ether.