Page 103 - Pharmacognosy-I (02-06-06-102)

2- If powdered clove or section in hypantium is mounted in
50% solution of caustic potash, the eugenol in the volatile
oil react to give needle shaped crystals of potassium
eugenate.

3-Thin layer chromatography (B.P. 2008).
Absorbent: silica gel GF 245
Solvent system: Toluene
Extraction: Powdered clove 0.1 g was shaken with 2 ml
dichloromethane
For 15 minutes, filter and evaporate to dryness on water bath
and
dissolve the residue in 2 ml toluene.
Reference: eugenol in toluene.
Visualization: under UV lamp.
Spray the chromatoplate with anisaldehyde for 5 to 10 min.

Tests for purity: Clove contains:
1- No starch granules (mother clove) cereals.
2- No irregular fibrous cells.
3- Not more than few sclereids with thick numerously pitted
walls and large lumen frequently filled with yellowish-
brown amorphous content.
4- No reticulate thickened vessels and no prisms of calcium
oxalate (clove stalk).

Assay: Determination of volatile oil by steam distillation

Clove Products Used For Adulteration:

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