Page 180 - Applied Pharmacognosy

Detectors in HPLC

How Detectors Work?

Bulk property detectors Solute property detectors

❑ measure property of mobile ❑ measure property of solute

phase; (refractive index, absent in mobile phase (UV

light-Scattering, dielectric absorbance, electrochemical,

constant, density). fluorescence, conductivity, IR

❑ Non-specific. absorbance).

❑ Example: Refractive index ❑ Specific to analyte chemistry.

detector. ❑ Example: UV-VIS absorbance

detector.

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