Page 175 - Applied Pharmacognosy
P. 175
Maintenance & Storage of Separation Column
How to Rinse the Column after HPLC Run?
❑ Use an eluent with a high elution strength:
✓ Reversed phase mode: Solution with a high proportion of organic
solvent.
✓ Ion exchange mode: Solution with a high salt concentration.
❑ Consider secondary retention effects:
✓ To remove basic substances from a reversed phase column: Use
an acidic solution and add an ion pair reagent.
✓ To remove hydrophobic substances from an ion exchange
column: Add an organic solvent.
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