Page 172 - Applied Pharmacognosy
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What is Inside the Column-The Stationary Phase
Ion Exchanger is another Possible Column Type
❑ Resin or silica gel is often used as a support covalently linked
with exchanger.
Resin Resin Resin
The exchange groups An eluent ion is The solute ion is driven
are in equilibrium with driven away away by an eluent ion
anions in the eluent. and a solute ion and is adsorbed by the
is adsorbed. next exchange group.
Solute ions and eluent ions compete for ion exchange groups.
If salt concentration of the eluent increases, solutes are eluted soo1n71er.
