Page 51 - Clinical Biochemistry 08PB804
P. 51
Figure 18: Serum HBV DNA level
Realtime PCR: Threshold Cycle
accumulation of PCR product
Fluorescent probes
Standard curve
Figure 19: Standard curve
50
40
30
20
10
0
123456
Concentration log 10
Role in HBV diagnosis and management:
• Staging / work up of chronic hepatitis B virus infection
• Response to therapy.
• blood safety (TMA; Transcription-mediated amplification)
CHB stage Serum HBV DNA (IU/mL) HBsAg log10 IU/mL
>2X106-7 4.5-5.0
Immune tolerant 2X104-5 3.0-4.5
Immune clearance <2X103 1.5-3.0
(HBeAg pos)
Inactive carrier
