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Viral load: Figure 24: HCV Conc.
RealTime PCR:
Sensitive as it can detect
LOD 5-15 IU/mL
Therapeutic monitoring: Med Microbiol Immunol. 2015; 204(4): 515–525
Important for Peg/IFN and Ribavirin:
Baseline and RGT
DAA monitoring?
• Baseline levels don’t affect duration of therapy
• Viral kinetics during therapy don’t necessarily predict outcome
HCV drug resistance testing:
Genotype specific PCR and Sanger sequencing, online analysis
Figure 25: specific PCR and Sanger sequencing
T54 Q80 S122 I132 R155 D168
V36 V55 V170
F43 Y56 A156 N174
NS3/4A M28 P58 Y93 A553
NS5A G554
Q30 S556
L31 G557
G558
L159 S282 C316 A395 N444 P495 D559
C289 V321 N411 C445 Y561 S565 I585
L326 S368 M414 Y448
C451 Lontok et al., 2015
NS5B
