Page 78 - Instrumental Analysis - Pharm D Clinical- 07-PA403

Application of HPLC (cont.)

Qualitative Quantitative
(tR, K’, α ) (peak area)

using
• External Standard

method

OR
• Internal standard method

Prof. Omayma Abdelrazak Amin 65

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External Standard method

- Calibration curve

- Regression equation Y = a + bX 66

Prof. Omayma Abdelrazak Amin

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