Page 76 - Instrumental Analysis - Pharm D Clinical- 07-PA403

6- Chiral chromatography

• Used for separation of the enantiomers
• Using chiral stationary phase

61

Choice of HPLC technique

• High MWt analytes → size exclusion chrom.
(e.g. biomolecules)

• Ionic analytes → ion exchange chrom.
(e.g. inorg. Cations & anions, ionizable compounds)

• Polar nonionic analytes →partition chrom.
(e.g. drugs)

• Nonpolar water insol. analytes →adsorption chrom.

62

62

31