Page 73 - Instrumental Analysis - Pharm D Clinical- 07-PA403
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3- Size exclusion chromatography
• separation is based on the molecular size of the
components of the mixture
• Molecules that are too large relative to the pores of
the porous packing material on the column elute
first
• Small molecules that enter the pores elute last
• e.g. for fractionation of protein analysis
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