Page 70 - Instrumental Analysis - Pharm D Clinical- 07-PA403
P. 70

Mobile phase dissolves and transports the analyte
Stationary phase retains the analyte by:

dissolving          adsorbing               reacting                         Sieving

Partition chromat.  Adsorption chromat.  Ion-exchange ch.                   size exclusion
                                         Affinity Chromat.                  chromat.
                                         Chiral Chromat.

    Inert support            mobile ph.  solid st. ph.
                    Liquid st. ph.
49
                                                                            49

1- Partition chromatography

 the st. ph. is a secondary liquid immiscible with the mo. Ph.
  held in place on the surface of finely divided solid (packing particles)

50

                                                                                                                25
   65   66   67   68   69   70   71   72   73   74   75