Page 67 - Instrumental Analysis - Pharm D Clinical- 07-PA403

The majority of HPLC applications used C8 and
C18 columns

• it is commonly used in the pH range of 2 – 8, due to:

- dissolution of silica at pH > 8

- hydrolysis of bonded phase at pH < 2

Prof. Omayma Abdelrazak Amin 43

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Guard Column

• introduced before the analytical column
• Packing composition similar to the analytical column
• of larger particle size
• To remove particulate matter and contaminants from

the solvents, so it increases the life of analytical
column

Prof. Omayma Abdelrazak Amin 44

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