Page 64 - Instrumental Analysis - Pharm D Clinical- 07-PA403

Ways for elution of the mobile phase

1. Isocratic elution:
A single mob.ph. of constant composition is
used

2. Gradient elution:
Two (or more) solvent systems that differ
significantly in polarity are employed. the
ratio between volumes of the two solvents
is varied continuously or in a series of steps

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gradient elution is applied to improve separation efficiency

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