Page 61 - Instrumental Analysis - Pharm D Clinical- 07-PA403
P. 61
• Classical peak tailing in reversed-phase methods is
most commonly caused by ionic interactions
between basic analytes and residual silanols
• This tailing can be avoided using an endcapped
column
• End-capping is a process whereby residual silanol
groups are treated to convert them to less polar
surface functional groups (e.g. trimethyl silyl
derivative).
Prof. Omayma Abdelrazak Amin 31
31
HP L C
High Performance Liquid Chromatography
Prof. Omayma Abdelrazak Amin 32
32
16
