Page 66 - Instrumental Analysis - Pharm D Clinical- 07-PA403

d. Column and stationary phases

Analytical columns

Commonly used columns are 10, 15 or 25 cm long, 4.6
mm inside diameter, and packed with 5-μm particles

Prof. Omayma Abdelrazak Amin 41

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Silica Based Stationary phase

Silica gel (Bare silica) bonded phase silica

Silanol gp

OH
OH

Siloxane gp OH

hydrophilic R = alkyl gp (e.g. C8, C18 )
42 or alkyl cyano or amino gp..

C8, C18 st. ph. are hydrophobic

Prof. Omayma Abdelrazak Amin 42

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