Page 69 - Instrumental Analysis - Pharm D Clinical- 07-PA403

LC-MS

• The combination of HPLC with a mass spectrometry
detector produces a very powerful analytical tool (LC-MS)

• MS detector can be used for:
1- Quantitation
2- Structure elucidation

of the analytes eluting from the HPLC column.

Prof. Omayma Abdelrazak Amin 47

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Common HPLC techniques
(Separation modes)

(based on the mechanism of retention on the st. Ph.)

1. Partition chromatography (Normal & Reversed phase) (L-L)

2. Adsorption chromatography (L-S)

3. Ion-exchange chromatography (L-S)

4. Affinity chromatography (L-S)

5. Size exclusion chromatography (L-S)

6. Chiral chromatography (L-S)

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